Questo sito utilizza cookie, anche di terze parti, per migliorare la tua esperienza e offrire servizi in linea con le tue preferenze. Chiudendo questo banner, scorrendo questa pagina o cliccando qualunque suo elemento acconsenti all'uso dei cookie.

CHIUDI

   
Forgot your password?
Register
 
Prodotti per il mercato Italiano
Saliva kits
Hypertension / Calcium Metabolism
Controls
Autoimmune Diseases
Diabetes Monitoring
Tumor Markers
Steroids Hormones
Fertility Proteic Hormones
Thyroid Monitoring
Circulating Immuno Complexes
Miscellaneous
X close

AmplideX FMR1 mPCR Kit (Metilazione)

AmplideX FMR1 mPCR Kit (Metilazione)

CODE 49442
DESCRIPTION AmplideX FMR1 mPCR Kit (Metilazione)

 

FRAGILE X METHYLATION TESTING
 
The AmplideX® FMR1 mPCR reagents employ an innovative PCR-only approach for the detection of methylation status in the FMR1 gene. These reagents reveal the extent of methylation of each individual allele in both male and female samples, and thus eliminate the need to run tedious and time consuming Southern blots.
 
Kit Features
 
  • Eliminates the need for Southern blot analysis
  • Amplifies and detects normal, intermediate, premutation and full mutation alleles
  • Accurately measures the methylation fraction for each FMR1 allele at higher resolution than Southern blot analysis
  • Provides increased sensitivity for the detection of lower abundance mosaic alleles*
  • Includes spike-in digestion and amplification controls to ensure high performance with every sample
  • Designed to complement the AmplideX® FMR1 PCR Reagents
 
Workflow Overview
 
Vedi FIGURA 1 alla base di questa videata

A more informative and convenient alternative to Southern Blot
 
Vedi FIGURA 2 alla base di questa videata
 
  • Spike-in controls normalize results and report the success of the digestion reaction for each sample
  • Alleles are detected in both the FAM and HEX channels. A comparison of peak heights in the two channels is used to determine the methylation fraction for each allele
  • The methylation status observed using mPCR is concordant with Southern blot analysis
 
AmplideX®FMR1 mPCR results correlate well with observed phenotype

Vedi FIGURA 3 alla base di questa videata
 
  • Observed phenotype: Low functioning female (65 IQ), anxiety, mood swings, and ADHD
  • Observed genotype: Full mutation allele identified using AmplideX® FMR1 PCR Kit and fully methylated status resolved using Amplidex® FMR1 mPCR Kit. The results were concordant with Southern blot and consistent with the observed phenotype.
 
 
Ordering Information

Vedi FIGURA 4 alla base di questa videata
 
*For Research Use Only. Not for use in diagnostic procedures.
Courtesy Rush University Medical Centre




Download FIGURA 1.pdf
FIGURA 2.pdf
FIGURA 3.pdf
FIGURA 4.pdf

DiaMetra srl Unipersonale

p.iva 12585340156
info@diametra.com

HEADQUARTER, R & D and MANUFACTURING

Via Pozzuolo, 14 - 06038 Spello - Perugia Italy
T +39.0742.24.851 +39.0742.30.19.29
F +39.0742.31.61.97
Management and Coordination : Immunodiagnostic Systems (IDS) Ltd, Boldon-UK
 
DiaMetra | © All rights reserved 2011