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Quantidex DNA Assay kit

Quantidex DNA Assay kit

CODE 49539
DESCRIPTION Quantidex DNA Assay kit

Introduction

Measures the DNA that matters: PCR-amplifiable DNA

Provides actionable guidance that rescues low-quality DNA and helps assure the accuracy of next-generation sequencing calls

A 2-in-1 assay: Distinguishes low-quality, unamplifiable DNA from DNA with sample-derived PCR inhibition

Simple, high-throughput workflow using a convenient multiplexed design

Ideal for quantifying challenging sample types like FFPE and FNA

Product descritpion

The QuantideX® DNA Assay is a multiplexed quantitative PCR assay that measures the absolute copy number of PCR-amplifiable DNA in a sample and reports PCR inhibition.

The QuantideX® Assay determines the functional quality of sample DNA using the QFI™ Score, which is the fraction of total genomic DNA copies that can be amplified. The QFI™ Score and copy number of amplifiable DNA provide actionable guidance that informs the input into NGS target enrichment and helps assure high analytical sensitivity and specificity. In addition, the QuantideX® Assay flags PCR inhibitors in the sample and provides an opportunity to salvage such samples through a subsequent clean-up step prior to further processing.

*For Research Use Only. Not for use in diagnostic procedures.

References:

  1. Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies. Sah et al. Genome Med 2013;5(8):77
  2. Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel. Choudhary et al. BMC Med Genomics (in press)





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