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AmplideX PCR/CE DMPK Kit

AmplideX PCR/CE DMPK Kit

CODE 49655
DESCRIPTION AmplideX PCR/CE DMPK Kit
 
The AmplideX PCR/CE DMPK Kit introduces a groundbreaking alternative for the detection and sizing of CTG trinucleotide repeats within the DMPK gene. Leveraging the Repeat-Primed PCR (RP-PCR) design in a simple, streamlined, PCR-only workflow, the kit provides an opportunity to replace Southern blot technology and put DMPK analysis within the reach of laboratories everywhere.
 
 
Feature and Benefits
 
AmplideX technology has revolutionized how repeat expansions within the FMR1C9orf72, and TOMM40 genes can be analyzed, significantly expediting time-to-result and reducing the reliance on cumbersome Southern blot analysis.  The AmplideX PCR/CE DMPK Kit extends this groundbreaking approach to the DMPK gene, which has long challenged laboratory analysis due to the presence of very large expansions (over 1000 repeats) and a high degree of mosaicism.  The AmplideX DMPK kit, which is for use on widely established CE systems, overcomes these challenges and enables the sensitive detection and sizing of these DMPK repeats all within a single laboratory shift – without the need for Southern blot confirmation.
 
 
Reduced Complexity
Analysis of the DMPK gene has been simplified through:
·         Proprietary PCR solutions for GC-rich amplification and detection
·         Eliminates need for Southern blot
·         Resolves zygosity and detects mosaicism
 
Optimized Workflow
Valuable operator hands-on time has been significantly reduced through:
·         Direct injection of PCR products into CE instruments
·         Fully kitted, end-to-end solutions that significantly reduce hands-on-time
·         Sample-to-result possible within a single shift
 
Quality Performance
Performing DMPK analysis with greater sensitivity and accuracy:
·         Accurate sizing of all alleles ≤200 CTG repeats and detection of all alleles >200 repeats using CE
·         Optional AGE protocol allows sizing of alleles between 200-2000 repeats
·         Converts raw base pair data into number of repeats
 
 
Analytical Characteristics
Analytical characteristics of AmplideX PCR/CE DMPK Kit:
·         High degree of concordance to Southern blot for large expansions 
·         Accurate sizing of expansions up to 200 repeats 
·         Clearly resolves zygosity 




Download Brochure_AmplideX PCR CE DMPK Kit.pdf

AmplideX PCR/CE DMPK Kit - Asuragen Molecular Products

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p.iva 12585340156
info@diametra.com

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T +39.0742.24.851 +39.0742.30.19.29
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Management and Coordination : Immunodiagnostic Systems (IDS) Ltd, Boldon-UK
 
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